Laser Scanning Microscope
The NESSIE microscope by MONSTR Sense Technologies is a first-of-its-kind laser-scanning microscope. The microscope rapidly raster scans the laser input over your sample, providing advanced hyperspectral images in seconds. For those working with a cryostat or otherwise fixed sample: the objective height is variable by up to 5″, and the sample area is very large. The NESSIE microscope is a standalone microscope that may integrate just about any laser-based measurement with high resolution imaging, and it is designed to integrate well with our BIGFOOT ultrafast spectrometer.
Laser-scanning microscope (LSM) & software
The input of NESSIE is a single laser beam, and the output is a counter-propagating beam collected from the excitation point of the sample. Our LSM hardware design ensures that the counter-propagating signal does not drift with respect to the input beam as the image is scanned. The NESSIE is therefore ideally suited for adding imaging microscopy to your laser-based experiment.
The software is both user friendly and flexible. We offer a LabVIEW-based software package that incorporates your metrics with our imaging control algorithms. This package generates images in real time and has controls to change the field of view or even fix the laser spot on a single point. We also offer APIs for writing your own code integrate NESSIE with your experiment.
For more information on the capabilities of NESSIE when combined with our BIGFOOT spectrometer, see the Related Products section above.
Fig. 1: Images of GaAs quantum well measured at room temperature using NESSIE. a) White light image measured with camera. b) Laser-scanned linear reflectance measurement with 80 MHz laser (5 mW laser output) tuned to GaAs bandgap. c) Simultaneously measured laser-scanned four-wave-mixing image reveals subsurface defects affecting the GaAs layer.
For integrating with your laser-based laboratory experiment or OEM applications, NESSIE is there. We designed the system to work with a wide range of laser wavelengths and needs.
Two-photon imaging microscopy
Insert NIR light from an ultrafast laser, and detect two-photon emission. Detection requires a short pass filter and a sensitive photodetctor or avalanche photodiode (APD).
Stimulated Raman imaging microscopy (and CARS)
Insert two combined light sources to probe Raman modes, and detect the emitted light using a spectrometer or spectrally filtered photodetector. Conventional SRS uses a narrow band pump and detects a differential pump-probe signal at the wavelength of the probe. For coherent anti-Stokes Raman (CARS), the anti-Stokes signal is blue detuned from the pump and probe. Detection requires a short pass filter and a sensitive detector.
Fluorescence lifetime imaging microscopy
Insert a pulsed light source and detect fluorescence emission using a fast photodetector or avalanche photodiode.
Transient absorption microscopy
Insert an ultrafast laser with a wavelength that is absorbed by the sample. Transient absorption uses either resonant pump and probe, or above resonant pumping, both with detection at the probe wavelength. Detection typically requires an amplified photodetector and lock-in amplification. For high sensitivity and well-integrated software functionality, consider using our BIGFOOT spectrometer to generate and detect the ultrafast pulse sequence.